Selected peptide-based fluorescent probes for biological applications

• Debabrata Maity

Beilstein J. Org. Chem. 2020, 16, 2971–2982, doi:10.3762/bjoc.16.247

• merocyanine (Mc), and gray sphere: spiropyran at a ring-closed state (Sp); colocalization experiments using peptide 14, LysoTracker Green DND-26 (LTG) and Hoechst 33258 in A549 cells. Cells were stained with c) 10 µM peptide 14 (channel 1: excitation: 515 nm, emission collected: 600–650 nm), d) 0.1 µM LTG

• (channel 2: excitation: 488 nm, emission collected: 500–550 nm) and e) 10 µg/mL Hoechst 33258 (channel 3: excitation: 405 nm, emission collected: 420–470 nm). Reproduced from [64], “A switchable peptide sensor for real-time lysosomal tracking”, © 2014 Chen et al., licensed under a Creative Commons

An overview of recent advances in duplex DNA recognition by small molecules

• Sayantan Bhaduri,
• Nihar Ranjan and
• Dev P. Arya

Beilstein J. Org. Chem. 2018, 14, 1051–1086, doi:10.3762/bjoc.14.93

• the past few years. Keywords: alkylators; antibiotic; anticancer; antineoplastic; antiproliferative; DNA recognition; groove binders; hairpin polyamides; Hoechst 33258; intercalators; Review 1. Introduction DNA is one of the central components of cellular machinery and storage unit of genetic

• ) was investigated in vitro by Shahabadi et al. by multispectroscopic techniques and molecular modeling study [108]. It has been shown that cytarabine acts in a groove-binding mode, which was confirmed by fluorescence experimental results of Hoechst 33258 displacement by the drug. Hydrophobic

• phosphate backbone and the binding affinity strongly depends upon the nature and length of the linkers. 2.2. Bisbenzimidazoles Bisbenzimidazoles are one of most extensively studied DNA minor groove binding compounds; Hoechst 33258 and 33342 are representatives of this class of compounds as shown in Figure

Synthesis of szentiamide, a depsipeptide from entomopathogenic Xenorhabdus szentirmaii with activity against Plasmodium falciparum

• Friederike I. Nollmann,
• Andrea Dowling,
• Marcel Kaiser,
• Klaus Deckmann,
• Sabine Grösch,
• Richard ffrench-Constant and
• Helge B. Bode

Beilstein J. Org. Chem. 2012, 8, 528–533, doi:10.3762/bjoc.8.60

• for 45 min at 28 °C (Molecular Probes, Invitrogen). Hemocyte monolayers were washed with 1× PBS and fixed with 4% paraformaldehyde for 15 min before permeabilizing with 0.2% Triton X-100 in PBS for 10 min. Cells were stained with FITC-conjugated phalloidin and Hoechst 33258 and finally washed with PBS

(Pseudo)amide-linked oligosaccharide mimetics: molecular recognition and supramolecular properties

• José L. Jiménez Blanco,
• Fernando Ortega-Caballero,
• Carmen Ortiz Mellet and
• José M. García Fernández

Beilstein J. Org. Chem. 2010, 6, No. 20, doi:10.3762/bjoc.6.20

• specifically to the minor groove of the DNA. A pentameric thymidyl DNG incorporating bis-benzimidazole (Hoechst 33258) ligand (64) was synthesised [105]. The stability of DNG-Hoechst conjugates 64 and 65 with a 30-mer double-strand DNA (dsDNA) and single-strand DNA (ssDNA) were evaluated. Fluorescent emission

• studies showed that hybridization of DNG-Hoechst conjugates 64 and 65 to dsDNA enhances the stability of the triple helix through simultaneous minor groove binding by the tethered Hoechst 33258 ligand. Furthermore, Hoechst 33258 is able to enhance the stability of the duplex DNG·DNA with a flexible minor